应用生物信息学预测牛新microRNA及验证

doi:10.11843/j.issn.0366-6964.2015.08.006

Abstract

Abstract:

This study aimed to predict novel miRNAs of bovine by comparative genomics and bioinformatics analysis.According to the conservation of miRNAs sequence，we compared the known miRNAs in human，mouse，sheep，pig and dog with the bovine genome sequence (UMD3.1) in NCBI to predict novel miRNAs，some of which were validated by qRT-PCR.A total of 44 novel miRNAs of bovine were obtained.Four of these were randomly selected to be validated.It was found that the 4 predicted miRNAs were expressed in mammary gland，cardiac muscle，uterus and liver of lactating Chinese Holstein cows.The methodology provides an alternative approach to predict the novel miRNAs and provide a groundwork for gene expression and trait formation mechanisms in dairy cattle.

CLC Number:

S823.2

CUI Xiao-gang，YANG Shao-hua，XIE Yan，ZHANG Sheng-li，ZHANG Qin，SUN Dong-xiao. Computational and Experimental Identification of Novel MicroRNAs in Bovine by Bioinformatics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1317-1324.

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Computational and Experimental Identification of Novel MicroRNAs in Bovine by Bioinformatics

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